Sweetpotato Virus Disease (SPVD) is a significant problem in sweetpotato cultivation globally, particularly in Sub-Saharan Africa. It results from the interaction of two viruses the whitefly-transmitted Sweetpotato Chlorotic Stunt Virus (SPCSV) and the aphid-borne Sweetpotato Feathery Mottle Virus (SPFMV). Currently, control methods are limited to sanitation and virus-free planting material. Due to the lack of natural resistance to SPVD in the sweetpotato crop genepool, crop wild relatives are a potential alternative source of resistance genes. To evaluate sweetpotato crop wild relatives for resistance to SPCSV and SPFMV 53 accessions of nine species belonging to the Batatas complex, the species most closely related to cultivated sweetpotato, were subjected to repetitive cycles of grafting with infected stem cuttings and subsequent enzyme-linked immunosorbent assay (ELISA) screenings. This subset contains four accessions showing consistent high levels of resistance to SPCSV.