Flooding poses a major challenge to rice cultivation, especially in lowland areas where rice is a staple food. The phenomenon of submergence can drastically reduce growth and yield. To address this, rice breeding programs focus on genetic traits that confer flooding tolerance, particularly the SUB1A allele of the SUB1 gene, which enhances rice survival under submergence for up to two weeks (Kato et al., 2014; Dar et al., 2018). This gene helps plants endure anaerobic conditions by regulating metabolic processes (Nishiuchi et al., 2012; Perata, 2018). Located on chromosome 9, SUB1 has been integrated into high-yielding varieties. The Rice Biodiversity Center for Africa (RBCA) team identified seven Oryza sativa accessions possessing the qSub1 allele through the genotyping of 593 accessions using the 1k RiCA mid-density panel.