Potato cyst nematodes (PCN – Globodera pallida and Globodera rostochiensis) are serious pathogens that cause significant yield reduction and economic loss globally. Nematodes are difficult to control and impossible to eradicate once the soil has been infested. Thus, genetic resistance to PCN is critical to prevent yield losses. Since South America is the center of diversity for PCN, potato accessions evaluated for their resistance to PCN in South America represent a valuable source for resistance breeding and provide useful alternative planting material particularly in smallholder production systems. The PCN resistance subset contains 16 accessions that were selected based on CIP data records ranging from 1977 to 1998. These accessions showed resistance (15 acc.) or an intermediate level of resistance (1 acc.) to Globodera rostochiensis.