Cassava accessions were planted in Palmira and assessed for HCN content in roots, of importance for direct consumption and industry. The results obtained indicate that the accessions held at the genebank vary in terms of HCN content. This subset represents the 10 accessions with the highest percentages and the 10 accessions with the lowest percentages of HCN content. These 20 accessions were selected by making a histogram of the distribution of HCN content values of 481 accessions out of the 6,643 total accessions in the cassava collection