The objective of this work was to evaluate the agronomic performance of seven Brachiaria humidicola accessions during the establishment stage, in order to select those with better adaptation to the conditions of acid soil and low fertility of the Santander region of Barrancabermeja and Magdalena Medio, Colombia.

21 plots were used, with a completely randomized design and three replications for each accession. The evaluations were made since the seventh day after the seeds were planted and the plots were considered established when the covered area reached 80%. The emergence, cover, height, lateral displacement, dry forage production, incidence of pests and diseases and nutritional deficiencies of the plants were determined; the bromatological analysis was also made.

The B. humidicola accessions had an average germination of 30%. The cumulative variance was 88,67% in the first three components. The total variability was concluded to be high, with regards to the measured and/or estimated indicators, which allowed the adequate differentiation of the accessions. The indicators of higher contribution in group formation and selection were: cover, dry matter, dry forage production, fat percentage, leafiness, energy and protein.